Cancer cell line tag (<100bp) Knock-in
spCas9 edited HCT116 cell pool next generation amplicon sequencing
Prime.io’s CasN technology and expertise enable us to identify one positive clone for every 40 clones screened, saving us from culturing and genotyping at least 100 additional clones to obtain a positive clone compare to the spCas9 technology.
Validated Knock-In Efficiency in CasN-Edited Single Cancer Cell derived Clone
Total 56.82% Knock-in Success Rate (2/4 alleles for target gene in client's cancer cells)
Cancer Cell Line Gene Knock-Out
Validated Knock-Out Efficiency in CasN-Edited Single Cancer Cell derived Clone
Total 97.67% Knock-out Success Rate (4/4 alleles for target gene in client's cancer cells).
Faster Knock-In/Out Project Turnaround
Deliver 7 weeks faster on average than other CROs
Our gene editing Service Procedure
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